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 Wysłany: Wto 5:59, 25 Sty 2011 Temat postu: ugg pas cher Hasty spy Sheep Disease Diagnosis and |
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Hasty spy Sheep Disease Diagnosis and Control
000; 2. Quality livestock development area of Jilin Province Horqin, Jilin Baicheng 137000; 3. White City ChaganHot Tourism Economic Development Town Heights veterinary service stations, Jilin Baicheng 137017) 【Key Words] s858.26 【literature standard iP, ~ IB 【Article ID] 1672-2078 (2007) 11-0044 A spy is a sudden O1 sheep Clostridium perfringens type C caused by sepsis, the disease in sheep, sudden death, peritonitis, and is characterized by ulcerative colitis, a serious harm to the production of infectious sheep industry. Now with the case of a point of diagnosis and treatment of animal husbandry, the report is as follows. 1 incidence and clinical symptoms in May 2007, a sheep farm there grazing incidence of sudden death point of the phenomenon. According to field investigation: there are 13 points of the grazing sheep herders, herds of sheep only to 1400, the incidence of households have see a sheep mortality and morbidity, mostly sheep and goats 1 and 2 years Biaoqing good sheep. The performance of sudden onset,[link widoczny dla zalogowanych], short duration, and some did not see the symptoms of sudden death. Sometimes found Bingyang off base, lying to, performance anxiety, weakness and spasms, death within hours. 2 pathological change of a few sheep died necropsy, lesions consistent. Severe congestive duodenum and jejunum mucosa, ulceration, and some sections can be seen ulcers, chest, abdomen, and pericardial effusion large, exposed to the air to form cellulose floc clot, film-like on a small point of bleeding. 3 3.1 bacteriology laboratory sterile body cavity effusion to the spleen a few pieces of diseased smear, Gram staining, to see the same stuff short and thick, rounded at both ends, alone, in pairs or in short chains of Gram-positive bacteria. Isolation and culture taking 3.2 diseased blood glucose agar was inoculated, and with the anaerobic culture method for bacterial isolation and culture, 37 ~ C cultured 24h, blood agar plates in the formation of small round, central uplift, white, opaque colonies around the hemolytic colonies. Pick a single colony staining,[link widoczny dla zalogowanych], and saw a shape above the Gram-positive bacteria. 3.3 pathogenicity test animals with severe inflammation of the intestine to the contents, add 1 to 3 times diluted with normal saline, centrifuge 3000r/min Received: September 7, 2007 centrifuge 5min, the supernatant, and then by season's filter filtration, the filtrate obtained venous inoculation in mice 5, 0.2mL / only,[link widoczny dla zalogowanych], and the other with 5 as the control mice, intravenous injection of saline. Experimental group mice after inoculation and all died within 10 ~ 60min, the control group survived without exception. Prove that the compound contain toxins taken ill there. 3.4 biochemical tests bacterium can break down glucose, sucrose, lactose, maltose, nitrate reduction, resulting in HS, Yang glycosides do not break down, do not produce indole, and violent fermented milk. Consistent with the characteristics of Clostridium perfringens. 3.5 sterile test tubes and test taking four, each tube 0.5mL of intestinal contents into the filtrate, and then were added in each tube B, C, D-type Clostridium perfringens antiserum 0.25mL, in Chapter 4 by adding normal saline only as control. While the four test tubes placed in the box at 37 ℃, 40min after the injection of mice of the two lines and found that anti-vaccination serum D-2 mice all died within 10min,[link widoczny dla zalogowanych], the other mice were healthy living. According to B-type anti-serum and in B, C, D type toxin, C-type anti-serum and in B, C-type toxin, D-antiserum could neutralize the characteristics of D-type toxins, the disease can be proved that the compound obtained for the C-type Clostridium perfringens exist. Comprehensive epidemiological investigation, clinical manifestations, pathology and laboratory test results analysis, confirmed the disease is caused by Clostridium perfringens type C sudden spy sheep disease. 4 4.1 isolation Bingyang control measures and strict disinfection. Abnormal performance in the sheep flock to immediately pick out isolated, dead sheep and destruction of sound processing, cleaning pens feces Yang She and feeding utensils poisoned with 0.1% solution of a hundred and complete disinfection. 4.2 All investment services in general 10% of the sheep lime solution, a large sheep 200mL / only, lamb 50 ~ 80mL / only, once a 3d. Sheep with no incidence of 4.3 Bingyang application of drug therapy. Shortness of the course of the disease,[link widoczny dla zalogowanych], treatment is often too late to have died. Longer duration of those with Clostridium welchii type C anti-serum treatment, or the application of antibiotics and sulfa drugs, with a strong heart, sedation and other symptomatic treatment can be achieved certain results. Head cents, then (143l a 87989877'868l4288 / 86814 {88E-mail: zhubian @ siall】 More articles related to topics：
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