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 Wysłany: Wto 10:17, 22 Lut 2011 Temat postu: Puhuang and Danshen on the formation of atheroscle |
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Puhuang and Danshen on the formation of atherosclerosis preventive effect
For the HS, HSP,[link widoczny dla zalogowanych], HSS and the NCS group. HS group, HSP and HSS group was transferred to serum cholesterol levels about the same value. Methods: The steps the procedure described by Hanss slightly modified,[link widoczny dla zalogowanych], 37 TCM Information l900 Qilu No. 2 (Total 2D vessel) serum culture growth in the second and then first 3,[link widoczny dla zalogowanych],7,10 days or stable growth in the first any day to join the cells 36 hours after the H-thymidine Jia Liepei nutrient solution on the (O.50el 【/ m1). After another 12 hours, cell adhesion to the glass slide and digested by pancreatic egg white, H a combination of radioactive thymidine into the cell DNA using liquid scintillation counter or glass fiber filter method of counting, CPM said. CPM, said the increase in the number of AC accretion. CPM said the same reduction in the number of inhibition. NCS group to take on the 3H binding to DNA of a number of mean thymidine (expressed as the mean PM) as the standard value (100 hiss), higher than the value of the results are interpreted as promoting the role of AsMc hyperplasia,[link widoczny dla zalogowanych], lower than the standard value is considered to be inhibited. Different kinds of serum on the role of AC by sacrifice, the white radiation imaging method can also be confirmed,[link widoczny dla zalogowanych], such as Fischer-Bzo-head u (1976) and Hanss (1979) described. Inhibition of serum lipid Salvia autoradiography only observed. Identify different groups of rabbits NJJs board with a modified Salzmam adhesive methods. Adhesion of platelets on glass beads morphological changes observed by electron microscopy to study. HS and NCS results in the AC Hyperplasia experiment was repeated three times and the results showed that: HS group than those of NCS CPM group, Wilcoxon tests confirmed that the tag line of this difference. Growth in the second and then the ninth day of culture (P <O.01) a very significant difference. HS group than those of most of the CPM NCSN the standard value (100) and, in the second cell culture growth on the ninth day, with x examination showed evidence of very different. HS, HSP and the NCS group in the role of AC results proliferation repeated 6 times and the results showed that: HSP group than in the HS group CPM, KrusRwlWallis tests confirmed the HS, HSP, and NCS Group 2 growth in cell culture (P <O .05) significant difference. HS group, the majority of CPM group than the standard value of the NCS (100 division), HSP group as the value of electricity. 38 These experiments also showed the growth of the 2nd culture cells before and after the ninth day of the growth rate reached a peak in the first 12 days started to decrease. In addition, HS group was significantly higher than the growth rate of HSP ~] q and the growth rate of NCS Group. That AC Puhuang inhibited the proliferation of culture. Autoradiography showed that the nucleus of isotope-labeled HS group was significantly higher than the percentage of NCS group P <O. 001 (Table 5). In addition, HSP group isotope labeling of the cytoplasm was significantly lower than HS group. P <0. O for a 0.0i, the HSP, HSS and did not differ between the NCS. NCS groups were observed under electron microscope on the glass beads are spherical and revolves with the platelet spreading, a small amount of pseudopodia. In the HSP group, platelet adhesion and the NCS group of the same shape. Hss platelets has been somewhat spherical, and a small amount of platelet pseudopod} HS in the aggregation state, were discoid, with a long: pseudopodia. Discussion of the 60s, many researchers report in the atheroma. AsMC important role, most recently from the in vivo and in vitro experiments confirmed that a proliferation of negative factors have accelerated the phenomenon of AC. . (Such as arterial hypertension, diabetes, caused by atherogenic food, Staphylococcus haemolyticus cable) in some anti-rheumatic drugs inhibit the effect of phenomena (such as dehydroepiandrosterone cortisone, aspirin, D a penicillamine , chloroquine). Typhae and Salvia has blood circulation, and reduce the clinical symptoms of angina. Early trials have shown that we Puhuang reduced rabbit serum cholesterol levels, reduce the size of atherosclerotic plaques. In this study, we demonstrated that Puhuang and Salvia also inhibited in vitro proliferation of all the weeks of AC 'knowledge, a strong stimulation of platelet AC releasing factor proliferation of different groups of adhesion; ael, morphological changes in board Puhuang provided to reduce platelet adhesion and Salvia aggregation can also reduce the role of platelet release factors. Recently, low-density fat egg white part of the report, said the LDL. Proliferation in vivo with the AC. Salvia is through the exchange of LDL Puhuang or inhibit the proliferation of the AC, or through direct inhibition of cell growth requires further study into the ~. (This article is taken from the English version of
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