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 Wysłany: Sob 22:33, 15 Sty 2011 Temat postu: DNA Single-and Double-Strand Break in Plasmid Indu |
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,[link widoczny dla zalogowanych]
DNA Single-and Double-Strand Break in Plasmid Induced by 80MeV / u 12C ^ 6 + Ion Irradiation
ateincellandorganism. OrganismCanadaptsomeofthesechanges,[link widoczny dla zalogowanych], butsomeofthesechangesareletha1. Besidebasedamage. DNAstrandbreaksarethemajorlesioninthegenomeduetoexposuretoionizingradiation. DNAstrandbreaksincludesingleanddoublestrandbreak. DNAdoublestrandbreaksfDSB) especiallyareconjecturedtobethemainlethaleventsincel1. ThePUC18plasmidWasusedinthestudy. TheDNAsingleanddoublestrandbreakinducedby12C6 + ionirradiationandtheirrelationshipwereinvestigatedanddiscussed. PUC a 18plasmidwasirradiatedwith80MeV/uC6 + deliveredbyHIRFL (HeavyIonResearchFacilityinLanzhou) atroomtemperature. Afterirradiation,[link widoczny dla zalogowanych], theagarosegelelectrophoresisf1.2% agarose, 150V, 0.5xTBE) wascarriedoutwiththeplasmid. Thenthroughfluorescencescanning, therelationshipetweenthe ~ equencyofsinglestrandbread (SSB), thefrequencyofdoublestrandbreak (DSB), theratioofDSBtoSSBanddosesweregottenasshowninFig. 1andFig. 2.0U insanity ∞ j ∞ declared Dose (Gy) Fig. 1DoseeffectcurvesforthefrequencyofSSBandDSB. . 2 scorpion Dose (Gy) Fig. 2DoseeffectCUlVeSfortheratioofDSBtoSSB. FromFig. 1andFig. 2, wefindmostofDNAstrandbreaksweresinglestrandbreaks (SSB),[link widoczny dla zalogowanych], whenthedoseislessthanorequalto160Gy. Therateofdoublestrandbreakswereincreasedwiththedoseincreasing. butwhenitgottoacertaindose, theDNAsingleanddoublestrandbreakswecandetectwerealldisappeared. Whenthedoseincreased,[link widoczny dla zalogowanych], theratioofsingleanddoublestrandbreakswerealsoincreased. However. itWasreportedthatthev-rayirradiationcouldinduceDNAdoublestrandbreakonlywhenthedosegottoseveralthousandGray. So, DNAdoublestrandbreaksweremoreeasilyinducedbyionirradiationthanby, y. rayirradiation.
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